The Cregan Lab
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More coming soon!

For reprints or current publication information, please contact Dr. Cregan.

These papers provide some good background information on what we do:

Kary Mullis' paper describing PCR can be found in:
Mullis, K.B. (1990). The unusual origin of the polymerase chain reaction.
Scientific American 262: 56-65.

The original papers on SSRs (microsatellites) as highly polymorphic genetic markers in humans:
Litt, M. and Luty, J.A. (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene.
American Journal of Human Genetics 44: 397-401.

Tautz, D. (1989) Hypervariability of simple sequences as a general source of polymorphic DNA markers.
Nucleic Acids Research 17: 6463-6471. Weber, J.L. and May, P.E. (1989).

Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction.
American Journal of Human Genetics 44: 388-396.

A paper describing the basics of SSRs in layman's terms:
Cregan, P.B. (1992). Simple Sequence Repeat DNA length polymorphisms.
Probe 2:18-22.

Basic procedures used in our laboratory for SSR marker development:
Cregan, P.B., Bhagwat, A.A., Akkaya, M.S., & Rongwen, J. (1994). Microsatellite fingerprinting and mapping of soybean.
Methods in Molecular and Cellular Biology 5: 49-61.

Early applications of SSR markers in soybean:
Morgante, M., Rafalski, A., Biddle, P., Tingey, S., & Oliveri, A.M. (1994). Genetic mapping and variability of seven Simple Sequence Repeat loci.
Genome 37: 763-769.

Jiang, Rongwen, Akkaya, M.S., Lavi, U., & Cregan, P.B. (1995). The use of microsatellite DNA markers for soybean genotype identification.
Theoretical and Applied Genetics 90:43-48.

Fluorescence-based SSR allele sizing (DNA profiling) in soybean:
Diwan, N., & Cregan, P.B. (1997). Automated sizing of fluorescent labeled simple sequence repeat markers to assay genetic variation in soybean.
Theoretical and Applied Genetics 95:723-733.

Song, Q., Quigley, C.V., Carter, T.E., Nelson, R.L., Boerma, H.R., Strachan, J., and Cregan, P.B. (1999). A selected set of trinucleotide simple sequence repeat markers for soybean variety identification.
Plant Varieties and Seeds 12:207-220.

Targeted Development of SSR markers:
Cregan, P.B, Mudge, J., Fickus, E.W., Marek, L.F., Danesh, D., Denny, R., Shoemaker, R.C., Matthews, B.F., Jarvik, T., & N.D. Young. (1999). Targeted isolation of simple sequence repeat markers through the use of bacterial artificial chromosomes.
Theoretical and Applied Genetics 98:919-928.

Cregan, P.B., Mudge, J., Fickus, E.W., Danesh, D., Denny, R., & Young, N.D. (1999). Two simple sequence repeat markers to select for soybean cyst nematode resistance conditioned by the rhg1 locus.
Theoretical and Applied Genetics 99:811-818.

Towards the Development of an integrated SSR map of the soybean genome:
Akkaya, M.S.,. Shoemaker, R.C., Specht, J. E., Bhagwat, A.A., & Cregan, P.B. (1995). Integration of simple sequence repeat (SSR) DNA markers into a soybean linkage map.
Crop Science 35:1439-1445.

Cregan, P.B., Jarvik, T., Bush, A.L., Shoemaker, R.C., Lark, K.G., Kahler, A.L., Kaya, N., VanToai, T.T., Lohnes, D.G., Chung, J., & Specht, J.E. (1999). An integrated genetic linkage map of the soybean genome.
Crop Science 39:1464-1490.

In addition to SSRs, Restriction Length Fragment Polymorphisms are commonly used genetic markers:
Shoemaker, R.C. & Specht, J.E. (1995). Integration of the soybean molecular and classical genetic linkage groups.
Crop Science 35:436-446.

Keim, P., Beavis, W., Schupp, J., & Freestone, R. (1992). Evaluation of soybean RFLP marker diversity on adapted germplasm.
Theoretical and Applied Genetics 85: 205-212.

Isozymes are another type of genetic marker being studied by USDA scientists:
Griffin, J.D., & Palmer, R.G. (1995). Variability of 13 isozyme loci in the USDA Germplasm collections.
Crop Science 32: 897-904.