Figure 7. RNA expression patterns for two ethylene receptor genes, TAE and NR, in tomato. RNA was extracted from ethylene treated and untreated stems (S), leaf abscission zones (A), petioles (Pt), proximal pedicles (P) pedicle abscission zones and green (G), breaker (B) and ripe (R) fruit. The same northern blot was first probed with 32P labeled TAE1 and then several months later with NR.

Figure 8. Effect of silver thiosulfate (STS) and auxin (IAA) on RNA accumulation of TAE1 and NR mRNA in leaf abscission zones from ethylene-treated tomato explants. IAA in lanolin paste was applied to the petiolar stump of explants at concentrations of 0 (- IAA) or 50 µM (+ IAA) 4 h prior to a 61 h exposure to ethylene. Explants were treated with sodium thiosulfate (- STS) or silver thiosulfate (+ STS) and exposed to ethylene for 88 h. After 61 or 88 h of 25 µL/L ethylene exposure for IAA or STS treated explants, respectively, RNA was isolated from leaf abscission zones (LAZ). Ten µg of total RNA were loaded per lane. The RNA blot was sequentially probed for mRNA encoding GUS, TAPG and TAE1.

Publications on ETR1 homologs eTAE1 and TFE27

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