THE mRNA FOR AN ETR1 HOMOLOGUE IN TOMATO IS CONSTITUTIVELY EXPRESSED IN VEGETATIVE AND REPRODUCTIVE TISSUES

Plant Molec Biol (1996) 30:1331-1338

Dingbo Zhou (1,2), Panagiotis Kalaitzis (1,3), Autar K. Mattoo (1) and Mark L. Tucker (1,4)

  1. Plant Molecular Biology Laboratory, USDA/ARS, Building 006, BARC-West, Beltsville, MD 20705, USA
  2. Department of Horticulture, University of Maryland, College Park, MD 20742, USA
  3. Molecular & Cell Biology Program, Microbiology Bldg. University of Maryland, College Park, MD 20742, USA
  4. Corresponding author

Abstract
Dominant mutations in the Arabidopsis ETR1 gene block the ethylene signal transduction pathway. The ETR1 gene has been cloned and sequenced (Science, 1993, 262: 539-544). Using the ETR1 cDNA as a probe, we identified a cDNA homologue (eTAE1) from tomato. eTAE1 contains an open reading frame encoding a polypeptide of 754 amino acid residues. The nucleic acid sequence for the coding sequence in eTAE1 is 74% identical to that for ETR1, and the deduced amino acid sequence is 81% identical and 90% similar. Genomic Southern blot analysis indicates that three or more ETR1 homologues exist in tomato. RNA blots show that eTAE1 mRNA is constitutively expressed in all the tissues examined, and its accumulation in leaf abscission zones was unaffected by ethylene, silver ions (an inhibitor of ethylene action) or auxin.

eTAE1 and related TFE27 cDNA sequences

eTAE1 expression relative to Nr expression in tomato Icon link to Figure of RNA blot

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