AN ETHYLENE-REGULATED DNA ELEMENT IN ABSCISSION-SPECIFIC GENE PROMOTERS AND THE EXPRESSION OF AN ETR1 HOMOLOGUE IN TOMATO ABSCISSION

In Kanellis A.K., (1997) Biology and Biotechology of the Plant Hormone Ethylene. Kluwer Academic Publishers, Dordrecht, Netherlands.

Tucker, M.L., Matters, G.L., Koehler, S.M., Zhou, D., Hong, S-B., Kalaitzis, P., Mattoo, A.K., Nath, P.

Abstract
Abscission correlates with the accumulation of cell wall hydrolases. Genomic clones for bean abscission cellulase (BAC), soybean abscission cellulase (SAC) and three polygalacturonase genes from tomato have been identified and sequenced. The region between -1 and -200 bp in the BAC promoter is highly conserved in the SAC gene. Particle gun bombardment of bean and stably transformed tomato indicate that the 210 bp BAC promoter confers tissue-specific and hormonal regulation. In addition, we have identified tomato cDNA clones, TAE1 and TFE27, complementary to putative ethylene receptor mRNAs. TAE1 and TFE27 mRNA expression is constitutive in leaf and flower abscission zones.

Publications reviewed in this symposium article:

  1. PG expression
  2. Bean abscission cellulase promoter
  3. Ethyelene receptor eTAE1
  4. Ethyelene receptor TFE27

Previously unpublished data:

  1. Relative expression of TAE1 and NR genes Icon link to RNA blot figure


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